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Customer Requirements

PCR Thermocycler

1. Steady state temp distribution using less than 15 Watts (1)
   • Prefer aprox. 2 Watts
   • Prefer No more than 5 watts in heating.
2. Reaches steady state in less than 10 minutes (2)
3. Runs off external power supply (3)
4. W/o computer hookups (use microcontroller)
5. Fully portable (2)
   • Fit within a cubic foot (ask Neil)
6. Operator interfaced with inputs for desired temperature for upper and lower limits. (1)
   • 80–95 deg C
   • 50 – 65 deg C
7. Region of interest stays optically visible top and bottom (1)
   • Wavelength =425–485
8. Slides have tolerance ± 0.5 mm all directions (1)
9. Slide must be interchangeable (1)
10. Prefer protected from convection environment (2)
    • Enclosed environment for glass slide
11. Variation from linear distribution must be ± 1 deg C (1)
12. Temperature across length is ± 0.5 deg C (1)
13. Temperature difference from set point is ± 0.5 deg C (1)
14. Middle region is optically visible (55 mm x 10 mm) (1)
15. Materials must be biocompatible. (1)
16. Ease of Use (“Idiot Proof”) (2)
17. Upper temperature range 80 – 95 deg C
18. Lower temperature range 50–65 ºC

(1) High Importance

(2) Medium Importance

(3) Low Importance

PCR Fluid Injector